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. 2018 Jul 1;11(7):3405–3415.

Figure 3.

Figure 3

FOXP1 overexpression can partially rescue miR-206-mediated inhibition of glioma cell proliferation. A. Venn diagram shows that in total of 73 targets were commonly predicted by TargetScan, TargetMiner, miRDB and picTar databases. B. qPCR analysis shows that FOXP1 is the most down-regulated gene among the 5 selected miR-206 potential downstream targets. C. Western blot shows that FOXP1 is down-regulated by miR-206. D. Sequence alignment of the FOXP1 3’UTR binding site of miR-206. E. Dual luciferase reporter assay shows that miR-155 can only down-regulate the wildtype FOXP1 3’UTR-containing reporter activity. F. Western blot shows that FOXP1 protein was up-regulated after FOXP1 overexpression in miR-206 stably overexpressed LN229 cells. G. The proliferation of miR-206 stably overexpressed LN229 cells can be partially rescued by FOXP1 overexpression. H, I. Edu incorporation of miR-206 stably overexpressed LN229 cells can be partially rescued by FOXP1 overexpression. (*: P<0.05; **: P<0.01; ***, P<0.001).