(A) AG490 reduces the proliferation of EC109 cells. EC109 cells were treated with different doses of AG490 (1, 10, 20, 30, 40, 50, 60, 70, 80, and 90 µmol/L) for 24 h. (B) AG490 reduces cell proliferation stimulated by NaHS in EC109 cells. EC109 cells were co-treated with NaHS (500 µmol/L) and different doses of AG490 (1, 10, 20, 30, 40, 50, and 60 µmol/L) for 24 h. (C) Effects of NaHS and AG490 on EC109 cell proliferation. EC109 cells were treated with NaHS, NaHS + AG490, and AG490 for 24 and 48 h. Cell number was counted directly under a microscope. AG490 reduced the NaHS-induced proliferation of EC109 cells (A and B). Data are expressed as mean ± SEM (n=3). **P<0.01 versus the control group. ++P<0.01 versus the NaHS group. C. **P<0.01 versus control group. ++P<0.01 versus NaHS group.