Figure 1.

IRI induces cardiac pyroptosis, inflammation, and miR-424 up-regulation. Hearts from individual mice were reperfused for 24 h. Control mice underwent a sham surgery (sham group) without IRI. (A) The evaluation of cardiac function was detected by echocardiography. (B) The parameters of cardiac function, including EF% and FS%, were significantly decreased (B), and LVEDD, LVESD, LVEDV and LVESV were analyzed. (C) The expressions of IL-1β and IL-18 at the serum and mRNA levels (D) in heart tissues were determined by ELISA and qRT-PCR, respectively. (E) Expression of cleaved caspase-1 and caspase-11 at the protein level were determined by western blotting. (F) The expression of miR-424 at the mRNA level was determined by qRT-PCR. Data are presented as the mean ± S.E.M. n=6. *P<0.05 & **P<0.01 versus sham group.