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. 2018 Jul 1;11(7):3222–3235.

Figure 2.

Figure 2

HRI induces cell pyroptosis and miR-424 up-regulation in H9C2 cells. After HRI, cell viability (A), MDA content (B) and SOD activity (C) of H9C2 cells were analyzed. (D) The proportions of apoptotic and necroptotic cells were analyzed by FACs. (D) The expressions of IL-1β and IL-18 at the protein level in culture medium and at the mRNA level (E) in H9C2 cells were determined by ELISA and qRT-PCR, respectively. (F) Expressions of cleaved caspase-1 and caspase-11 at the protein level were determined by western blotting. (G) The expression of miR-424 at the mRNA level was determined by qRT-PCR. Data are presented as the mean ± S.E.M. n=4. *P<0.05 & **P<0.01 versus control group.