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. 2018 Jul 1;11(7):3222–3235.

Figure 3.

Figure 3

miR-424 directly targets CRISPLD2 in IRI hearts and H/R-challenged H9C2 cells. A. The expression of FoxO3a at the protein level in IRI heart tissues and H9C2 cells subjected to HRI was determined using western blotting. B. Alignment of the miR-424 sequence with that of the 3’-UTR of CRISPLD2. C. CRISPLD2 3’-UTR construct mutated at the predicted miR-424 binding site. D. Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of the CRISPLD2 3’-UTR containing the miR-424 binding sites in the control, miR-424 mimic, and miR-424 inhibitor groups. E. The expression of miR-424 and CRISPLD2 at the protein level in the control, H/R, H/R + miR-424 mimic, and H/R + miR-424 inhibitor groups. F. The expression of CRISPLD2 at the protein level in the control, H/R, H/R + miR-424 mimic, and H/R + miR-424 inhibitor groups. Data are presented as the mean ± S.E.M. n=4; *P<0.05 versus control group; #P<0.05 versus H/R group.