Figure 5.

miR-424 regulates pyroptosis in IRI heart tissues via targeting CRISPLD2. (A) The expression of CRISPLD2 at the protein and mRNA levels in the control, I/R, I/R + miR-424 inhibitor, and I/R + miR-424 inhibitor + AAV9-siCRISPLD2 groups. (B) The evaluation of cardiac function was detected by echocardiography. The parameters of cardiac function, including EF%, FS%, LVEDD, LVESD, LVEDV and LVESV, were analyzed. (C) Expression of IL-1β and IL-18 at the serum and mRNA levels (D) in heart tissues were determined by ELISA and qRT-PCR, respectively. (E) Expressions of cleaved caspase-1 and caspase-11 at the protein level were determined by western blotting. Data are presented as the mean ± S.E.M. n=5; *P<0.05 versus sham group; #P<0.05 versus I/R group.