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. 2018 Sep 1;11(9):4374–4384.

Figure 2.

Figure 2

miR-223 directly targeted SSRP1. A. A representative diagram of the predicted wild-type (WT) or mutant (Mut) binding site of miR-223 in the 3’-untranslated region (UTR) of SSRP1 mRNA. The luciferase reporter plasmid containing the WT or Mut SSRP1 3’-UTR was co-transfected into 5-8F cells with miR-223 mimics. The luciferase activity of the cells was assayed at 48 h after transfection, and the values were normalized to the normal control values. *P < 0.05 (compared with the control). B. Western blot analysis examined the effects of three siRNAs and the negative control on SSRP1 expression. C. A qRT-PCR analysis revealed the effects of SSRP1 siRNA-810 and miR-223 mimics on the expression level of miR-223. D. A Western blot analysis revealed the effects of SSRP1 siRNA-810 and miR-223 mimics on the expression level of SSRP1. Error bars represent ± S.E. and *P < 0.05 versus control and NC. &P < 0.05 versus miR-223 group.