Figure 2.

HIF-1α and BAG3 were up-regulated under hypoxic condition and HIF-1α cannot regulate BAG3 expression. A. HIF-1α and BAG3 mRNA expression level in U87 glioblastoma cell line under hypoxic and normoxic conditions. Hypoxic group was induced by CoCl₂ (200 μM) and normoxic group was not treated with CoCl₂. B. Relative expression level of HIF-1α and BAG3 mRNA in U87 glioblastoma cell line under hypoxic condition. C. HIF-1α and BAG3 protein expression level in U87 glioblastoma cell line under hypoxic and normoxic condition. D. Relative expression level of HIF-1α and BAG3 protein in U87 glioblastoma cell line under hypoxic condition. E. BAG3 mRNA cannot be regulated by HIF-1α in U87 glioblastoma cell line. Cells were transfected with cDNA3-HIF-1α plasmid or cDNA3 plasmid for control. F. Relative expression level of BAG3 mRNA in U87 glioblastoma cell line. G. BAG3 protein cannot be regulated by HIF-1α in U87 glioblastoma cell line. H. Relative expression level of BAG3 protein in U87 glioblastoma cell line. I. BAG3 protein cannot be regulated by HIF-1α in U251 glioblastoma cell line. J. Relative expression level of HIF-1α and BAG3 protein in U251 glioblastoma cell line. Protein expression level was tested by western blot and mRNA expression level was tested by RT-PCR. Each experiment was repeated three times. Protein and mRNA expression level were normalized relative to the amount of GAPDH. Data were showed as mean ± SD. *P < 0.05 represented statistical significance.