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. 2018 Sep 1;11(9):4305–4318.

Figure 3.

Figure 3

Down-regulation of BAG3 decreased HIF-1α protein expression level in glioblastoma. A. Fluorescence micrographs showing U87 and U251cells transfected with EGFP and EGFP-shBAG3 plasmid (original magnification, 100 ×). B. Immunofluorescent micrographs of U87 glioblastoma cell line (original magnification, 100 ×). C. HIF-1α mRNA expression level in U87 glioblastoma cell lines transfected with EGFP-shBAG3 or EGFP plasmid. D. Relative expression level of HIF-1α mRNA in U87 glioblastoma cell line with down-regulation of BAG3. E. HIF-1α mRNA expression level in U251 glioblastoma cell lines transfected with EGFP-shBAG3 or EGFP plasmid. F. Relative expression level of HIF-1α mRNA in U251 glioblastoma cell line with down-regulation of BAG3. G. Down-regulation of BAG3 decreased HIF-1α protein expression level in U87 glioblastoma cell line both under normoxic and hypoxic conditions. H. Relative expression level of HIF-1α protein in U87 glioblastoma cell line with down-regulation of BAG3 under normoxic and hypoxic conditions. I. Down-regulation of BAG3 decreased HIF-1α protein expression level in U251 glioblastoma cell line both under normoxic and hypoxic conditions. J. Relative expression level of HIF-1α protein in U251 glioblastoma cell line with down-regulation of BAG3 under normoxic and hypoxic conditions. Protein expression level was tested by western blot and mRNA expression level was tested by RT-PCR. Each experiment was repeated three times. Protein and mRNA expression level were normalized relative to the amount of GAPDH. Data were showed as means ± SD. *P < 0.05 represented statistical significance.