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. 2018 Sep 1;11(9):4305–4318.

Figure 4.

Figure 4

Down-regulation of BAG3 inhibits proliferation of glioblastoma. A. CCK8 results of U87 glioblastoma cell line. U87 cells were double-transfected with pcDNA3/pEGFP, pcDNA3/pEGFP-shBAG3 or pcDNA3-HIF-1α/pEGFP-shBAG3. Absorbance values (A450) were detected at 12, 24, 48 and 72 hours after plating into 96-well plate. Data were showed as mean ± SD. B. CCK8 results of U251 glioblastoma cell line. C. Proliferation fold change of U87 and U251 glioblastoma cell lines at 72 hours after plating into 96-well plate. D. EdU assay result of U87 glioblastoma cell line. Double-transfected U87 cells were stained by DAPI or EdU 24 hours after plating into 6-well plate. EdU-positive cells were stain as red. Representative images were shown (original magnification, 200 ×). E. EdU assay result of U251 glioblastoma cell line. Double transfected U251 cells were stained by DAPI or EdU 24 hours after planted into 6-well plate. Representative images were shown (original magnification, 200 ×). Data were showed as means ± SD. *P < 0.05 represented statistical significance.