Figure 5.

Down-regulation of BAG3 promotes apoptosis of glioblastoma. A. Flow cytometry results of U87 and U251 glioblastoma cell lines. Cells were double-transfected and planted into a 6-well plate and apoptosis was detected after 48 hours of culture. B. Percentage rate of apoptosis. C. The expression of apoptosis-associated proteins in BAG3 down-regulated U87 glioblastoma cells. Cells in experimental group were transfected with EGFP-shBAG3 plasmid, and control group was transfected with EGFP plasmid. D. Relative expression level of apoptosis-associated proteins in U87 glioblastoma cells. E. The expression of apoptosis-associated proteins in BAG3 down-regulated U251 glioblastoma cells. F. Relative expression level of apoptosis-associated proteins in U251 glioblastoma cells. G. Reverse experiment of apoptosis-associated protein expression of U251 glioblastoma cells. Cells were double-transfected with plasmids. Protein expression level was tested by western blot. Each experiment was repeated three times. Protein and mRNA expression level were normalized relative to the amount of GAPDH. Data were showed as means ± SD. *P < 0.05 represented statistical significance.