Figure 3.

A. siRNA- is an siRNA that blocks TLR3 from being transfected into cells, and siRNA+ is a random protein siRNA transfected into cells, and water is transfected into cells with water instead of siRNA. Using software analysis, the expression level of TLR3 in siRNA+ was reduced by more than 70% relative to the level in siRNA- and water. B. The Western blot result of the expression level of PD-L1 after 72 hours of HCMV infection in U87, primary glioma, and astrocytes. C. The result of first analyzing the Western blot image using IMAGE J, and the obtained result was statistically analyzed using SPSS. In the U87 and primary glioma groups, the expression levels of PD-L1 in the TLR3+ and TLR3-groups were statistically significant, P < 0.05. In the astrocyte group, this difference was not significant.