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SIRT1 inhibited adipocyte differentiation of 3T3-L1 cells. A. The expression levels of SIRT1 mRNA in 3T3-L1 cells transfected with Vector, SIRT1, Scramble, and shSIRT1 were examined by RT-qPCR. B-F. Analyses of the PPARγ, C/EBPα, and FABP4 proteins by WB at 8 days after the start of differentiation. G. The quantification of TG was performed using the TG Content Assay Kit. *P < 0.05, compared with Vector or Scramble group.
