The proportion of Tfh cells in AP patients increased. The ratios of Tfh cells in peripheral blood of AP patients (n=35) and HCs (n=20) were detected by flow cytometry. A. Representative flow cytometry figure of the ratio of CXCR5+ cells in CD3, CD4 double positive cells; B. Statistical analysis of ratio of CXCR5+ cells in CD3, CD4 double positive cells; C. Representative flow cytometry figure of the ratio of CXCR5+ICOS+ cells and CXCR5+ PD-1+ cells in CD3, CD4 double positive cells; D. Statistical analysis of ratio of CXCR5+ICOS+ cells in CD3, CD4 double positive cells; E. Statistical analysis of the ratio of CXCR5+PD-1+ cells in CD3, CD4 double positive cells. Results are expressed as mean ± SEM, NS: no significant difference, *, P < 0.05; **, P < 0.01; ***, P < 0.001, ****, P < 0.0001.