Skip to main content
. 2018 Dec 1;11(12):5830–5839.

Figure 2.

Figure 2

miR-3646 promotes TNFα secretion in THP-1 cells potentially by suppressing RHOH. A. Semi-quantitative RT-PCR to determine the expression of miR-3646 in THP-1 cells transfected with miR-3646 mimic compare with control. B. Quantitative RT-PCR to detect the expression of miR-3646 in THP-1 cells transfected with miR-3646 inhibitor compared with control. C. The protein levels of TNFα in supernatant of cells with indicated treatment, determined by western blot analysis. D. Schema of the miR-3646 binding site within the RHOH 3’UTR. The mutated site is shown below. E. The psiCHECK-2 sensor plasmids containing the RHOH 3’UTR or mutated 3’UTR were co-transfected in 293T cells with miR-3646 mimic, or control mimic. Overexpression of miR-3646 decreased the luciferase expression compared to controls. No downregulation of luciferase was observed for the negative control or mutated plasmid with miR-3646 overexpression. F. Western blot analysis to determine RHOH protein expression in THP-1 cells transfected with miR-3646 mimic or control mimic. G. Western blot to determine the protein level of RHOH in THP-1 cells transfected with RHOH siRNA or control siRNA. H. Determination of TNFα expression in THP-1 cells transfected with RHOH siRNA or control siRNA. **P < 0.01 versus control.