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. 2018 Dec 1;11(12):5830–5839.

Figure 3.

Figure 3

miR-3646 promotes cell proliferation and migration of VSMCs. (A) Quantitative RT-PCR to determine the expression of miR-3646 in VSMCs treated with 20 ng/ml of PDGF-bb for 0, 24, 48 and 72 h. (B) Semi-quantitative RT-PCR to determine the expression of miR-3646 in VSMCs transfected with miR-3646 mimic or control mimic. (C) CKK8 assay to evaluate the proliferate rate of VSMCs transfected with miR-3646 mimic or control mimic. (D) Determination of cell cycle proteins CDK2, Cyclin D1, p21, and p27 in VSMCs transfected with miR-3646 mimic or control mimic. (E and H) Wound healing assay (E and G) and migration assay (F and H) to determine the and migration abilities of VSMCs transfected with miR-3646 mimic or control mimic. Cells invading or migrating to the bottom side of the upper chamber were stained with crystal violet, dissolved with acetic acid and quantified at OD 570 (H). **P < 0.01 versus control.