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. 2018 Dec 1;11(12):5830–5839.

Figure 4.

Figure 4

Silencing of RHOH restores cell proliferation and migration of VSMCs. (A) Western blot analysis to evaluate the protein levels of RHOH in VSMCs transfected with miR-3646 mimic or control mimic. (B and C) Quantitative RT-PCR (B) and western blot (C) to determine the expression of RHOH in VSMCs treated with 20 ng/ml of PDGF-bb for 0, 24, 48 and 72 h. (D) The expression of RHOH in VSMCs transfected with RHOH siRNA or control siRNA. (E) CKK8 assay to evaluate the proliferation rate of VSMCs transfected with RHOH siRNA and control siRNA. (F and G) Migration assay to determine the migration abilities of VSMCs transfected with RHOH siRNA and control siRNA. (H) Schematic view to present the regulatory role of the miR-3646-RHOH axis in CAD. *P < 0.05, **P < 0.01 versus control.