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. 2018 Dec 1;11(12):5901–5908.

Figure 4.

Figure 4

Validation of the relationship between miR-129-3p and BZW1. (A) The position of the miR-129-3p target site in BZW1 3’UTR and its conservation among species. The figure is from the TargetScan website (www.targetscan.org). (B, C) Expression of BZW1 in cancer cells after transfection with miR-129-1-3p or miR-129-2-3p detected by qPCR (B) and western blotting (C). A1 is the NC group, and A2 is the miR-129-1-3p mimic group; B1 is the NC group, and B2 is the miR-129-2-3p mimic group. (D) Luciferase reporter assays for SKOV3 cells transfected with pRL-TK vectors carrying BZW1 wild type (WT) or mutant (MUT) 3’UTR in the absence or presence of miR-129-3p mimics. Data are expressed as the mean ± sd. from three independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.001; compared with NC group.