Figure 5.

GO stimulates HONE1 cells to produce autophagosome. 50 μg/ml of GO and an equal amount of FBS-free medium were co-cultured with HONE1 cells for 6 h as a negative control and subjected to a series of treatments and observed by transmission electron microscopy. The GO-treated cells showed a large number of autophagosomes with a bilayer membrane vesicular structure. (A. TEM: 10000× in control group; B. TEM: 40000× in control group; C. TEM: 10000× in GO group; D. TEM: 40000× cell nuclei in GO group. The red arrow refers to the autophagosome).