miR-30b-5p expression was decreased and addition of miR-30b-5p inhibited EMT, migration, and invasion in prostate cancer cells. A. The expression of miR-30b-5p was investigated in DU145 and PC-3 cells compared with PrEC by qRT-PCR. B. The alteration of miR-30b-5p was investigated in DU145 and PC-3 cells with miR-30b-5p mimic transfection compared with NC by qRT-PCR. C, D. The expression of EMT-related E-cadherin, vimentin, and fibronectin was analyzed in DU145 and PC-3 cells with miR-30b-5p mimic transfection compared with NC by WB. E, F. Cell migration and invasion abilities were detected in DU145 and PC-3 cells with miR-30b-5p mimic transfection compared with NC. Data re the mean ± standard deviation from three independent experiments. *P<0.05.