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. 2018 Dec 1;11(12):5840–5849.

Figure 2.

Figure 2

Melatonin alleviated apoptosis and activated PI3K/Akt pathway in H9C2 cells exposed to hypoxia. H9C2 cells were pretreated with melatonin 100 μM for 2 h prior exposure to hypoxia for 48 h. A. Representative western blotting of Bcl2, Bax, and cleaved caspase 3 with β-actin determined as the internal control. B-D. Histogram representing relative protein levels of Bcl2, Bax, and cleaved caspase 3. E. Phosphorylation of PI3K and Akt as analyzed by immunoblotting. F, G. Relative levels of p-PI3K versus total PI3K and p-AKT versus total AKT as determined by blot densitometry. Data are shown as the means ± SEM (n=3). *P<0.05 compared with control group, #P<0.05 compared with hypoxia group.