Figure 5.

Effect of IrSPI on vertebrate host coagulation. (A) Thrombin generation (in fluorescence unit/s) triggered by adding 10 mM CaCl2 to platelet-poor plasma containing tissue factor and phospholipid (PPP-reagent). Curves in black were obtained in the presence of 0.8 µM IrSPI in refolding buffer, curves in orange in refolding buffer only, and curves in green in 0.15 M NaCl. (B) Simultaneous recording of the fibrin clot formation detected through the increase of turbidity at 405 nm; legend as in (A). (C) Fibrin clot formation was triggered as in (A,B) except that 6 nM tPA was added before clot formation. Half-lysis times (defined as the time to halve maximum turbidity) were not significantly different in the presence (curve in black) or absence (curve in orange) IrSPI. Results presented are mean of two independent experiments.