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. 2019 Oct 12;7(4):148. doi: 10.3390/vaccines7040148

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Impact of IrSPI on murine splenocyte proliferation. The impact of IrSPI on the proliferation of T and B cells subsets was evaluated in splenocytes of OF1 mice following mitogenic stimulation by Concanavalin A. Cell proliferation was measured by CFSE dye dilution and flow cytometry. Splenocytes from each of three mice were separately evaluated in triplicate. Results are expressed for each condition as the mean of triplicate wells for each condition. Successive peaks, displaying diminished CFSE labeling, reveal successive cell divisions.