Figure 8.

Antibodies elicited post vaccination in the PreUSSR-Vac2x ferrets were detected earlier and had increased neutralization activity compared to other experimental groups. Serum was collected at early time points after primary vaccination (Days 0, 3, 7, and 14 post vaccination). (A) HAI assays were performed as previously described using sera isolated from blood collection post vaccination against the Cal/07 (pH1N1) virus to determine the dynamics of antibody generation after vaccination. (B) Microneutralization assays were performed by standard protocol on MDCK cells using serum from all experimental groups post vaccination and prior to challenge. For simplicity, only the PreUSSR-Vac2x, PreUSSR-MockVac, Naïve-Vac2x and Navie-Mock Vac groups are shown. Student’s t-test was conducted to compare results between each group, * p < 0.05.