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. 2019 Dec 13;8(12):601. doi: 10.3390/plants8120601

Figure 2.

Figure 2

Overview of the basic flow of the CRISPR/Cas9 system in plant genome editing. 1. Select a genomic target where the mutation is to be introduced. 2. Design the sgRNA complementary to the expected target sequence. 3. The sgRNA and Cas9 under suitable promoters are cloned in plant binary expression vectors. 4. The components of the CRISPR/Cas9 system construct are delivered into the plants, via Agrobacterium-mediated transformation or particle bombardment. 5. The mutations in regenerated transgenic plants are identified using restriction enzyme assays and sequencing. 6 and 7. The removal of the CRISPR/Cas9 cassettes is possible in subsequent generations of plants.