Figure 3.

Comparison of two different resuspension volumes for quantification of serum bactericidal antibody (SBA) by ATP. Pooled anti-sera from mice immunized with N. gonorrhoeae P9-17 OMVs was tested for SBA to N. gonorrhoeae P9-17. Data are combined means ± SD from three independent experiments with 50 µL (ATP-50) or 25 µL (ATP-25) of re-suspended bacteria used to quantify ATP levels. The correlation between the two volumes was calculated using a two-tailed Pearson coefficient (p < 0.0001) and significant differences between paired sets of values were determined by multiple t-tests.