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. 2019 Nov 21;7(4):191. doi: 10.3390/vaccines7040191

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of SBA data obtained using CFU or ATP methods to quantify bacteria. Raw ATP (luminescence) values and colony counts obtained in an assay for SBA (A) and a comparison of SBA titres using CFU and ATP methods (B). Serial twofold dilutions of murine serum raised to OMV extracts from N. gonorrhoeae P9-17 were incubated with live N. gonorrohoeae P9-17. Data are combined means ± SD from three independent experiments. ATP values were scaled to the left axis and CFU values to the right axis in (A). The correlation between CFU and ATP values was calculated using a two-tailed Pearson coefficient. Differences between matched sets of CFU or ATP values were determined using multiple t-tests. * p < 0.05.