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. 2019 Nov 21;7(4):191. doi: 10.3390/vaccines7040191

Figure 6.

Figure 6

Reduction of serum or SBA sensitivity or after sialylation with cytidine-5′-monophospho-N-acetylneuraminic acid (CMP-NANA). Mid-log broth subcultures of N. gonorrhoeae MS11 (A) or FA1090 (B) were incubated with/without the addition of 4 μg/mL CMP-NANA and samples taken to detect bacterial ATP. The P9-17 strain (C) was incubated under the same conditions with the addition of murine anti-P9-17 OMV anti-sera at a dilution of 1/64 to examine the effect of sialylation on SBA. Data are combined means + SD from three independent experiments; statistically significant differences in sensitivity were determined using multiple t-tests with the Holm–Šidák method applied (A,B) or a paired t-test (C). Values were reported as zero in experiments where bacteria numbers had increased in the presence of NHS. * p < 0.05; ** p < 0.01.