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Expression of apoptotic proteins. Cleaved caspase-3 (A,B) were expressed in cell lysates and EVs. At 72-h post-inoculation, protein was detected via dot blot analysis and ELISA. Samm50 (C) presence was observed in cell lysates using dot blot analysis. Experiments were performed using four or five independent replicates. Statistical significance is indicated by (***) p ≤ 0.001.
