Figure 3.

Antibody response after single-shot vaccination. Mice were immunized with 10¹⁰ virus particles of recombinant Ad5-Mosaic, Ad5-A/PR/8/34, Ad5-Pdm09, Fluzone, or PBS and sera harvested three weeks later (n = 5). ELISA were performed with recombinant HA protein from influenza strains (A) A/CA/07/09 (Pdm09), (B) A/PR/8/34, (C) A/NC/20/99, and (D) A/Brisbane/59/07. Responses greater than two times the geometric mean titer (GMT) were considered positive. Data is shown as the Log2 mean with standard error (data was analyzed with one-way ANOVA and Bonferroni multiple comparison and compared to the Ad5-mosaic; **** p < 0.0001, ** p < 0.01).