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Ligand binding of PR-10 proteins. Indirect measurement of ligand binding by PR-10 proteins via the 8-anilino-1-napthalenesulfonic acid (ANS) displacement assay. Binding is measured by percent increase or decrease of the fluorescent signal of the ANS-protein complex (40 uM ANS-40 uM protein) with no competitor and recorded as a percentage of the baseline signal on the y-axis. The x-axis shows the concentration of competitor ligand in uM.
