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. 2019 Nov 8;8(4):214. doi: 10.3390/antibiotics8040214

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PlyGVE2-derived recombinant lysins schematics and SDS-PAGE. (A) Schematics, PlyGVE2 supplied the N-terminal CAT domain (green), GVE2_CAT, and the C-terminal CWB domain (blue) comes from one of several C. perfringens endolysins (PlyCP10, PlyCP18, PlyCP33, PlyCP41, PlyCP26F). Black box indicates the C-terminal 6 x His domain. GVE2_CAT-CP26F_CWB * is the previously published lysin PlyGVE2CpCWB. (B) SDS-PAGE of GVE2_CAT fusion proteins purified by nickel chromatography. Lane 1 is Precision Plus Protein standard (Bio-Rad). The other lanes are as follows: lane 2, GVE2_CAT; lane 3, GVE2_CAT-CP10_CWB; lane 4, GVE2_CAT-CP18_CWB; lane5, GVE2_CAT-CP33_CWB; lane 6, GVE2_CAT-CP41_CWB; lane 7, GVE2_CAT-CP26F_CWB; lane 8, PlyGVE2.