Expression of EMT markers in NPC cells (a) (c) Western blot assay was performed to detect the expression of ZO-1, N-cadherin, E-cadherin, vimentin, and GAPDH in CNE1 and CNE2 cells after treatment with dose-dependent rhHMGB1. Each experiment was performed in triplicate. (b) (d) Quantitative analyses of expression of EMT markers in CNE1 and CNE2 cells. Data are expressed as mean ± SD, *P < 0.05. The data represent the average of three experiments. rhHMGB1: recombinant human high mobility group box 1; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.