Figure 1.

Liver morphological and histological characteristics. (a) Paraffin-embedded liver sections stained with hematoxylin and eosin (H–E) (magnification: ×200). (b) Paraffin-embedded liver sections stained with periodic acid–Schiff (PAS) (magnification: ×200). (c) Scores of the nucleus and cell structure, cell arrangement, macrophage infiltration, and glycogen deposition. Each bar or dot and error bar represents the mean±SD (n = 10).

Rats consumed high-fat diets supplemented with (1) 50% ethanol blackberry leaf (450 mg/kg bw; BL), (2) 50% ethanol blackberry fruit extract (450 mg/kg bw; BF), (3) the mixture of blackberry leaf and fruit extracts (2:1; 150 mg/kg bw; BLF), (4) dextrin (450 mg/kg bw; NAFLD-C), and (5) milk thistle extracts (150 mg/kg bw; positive-control) for 12 weeks. Rats had low-fat diets (20 En% fat diet) containing 450 mg dextrin/kg bw as the normal-control group. ^a,b,c,d Bars with different letters were significantly different by Tukey’s test at P < 0.05. (A color version of this figure is available in the online journal.)