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. 2019 Dec 9;6(4):114. doi: 10.3390/medicines6040114

Figure 1.

Figure 1

Proteomic analysis of HaCaT cells using reverse phase protein array (RPPA) technology. (A) Unsupervised and supervised heatmaps from RPPA analysis on HaCaT cells treated with the following agents: Control (DMSO only), EGF (100 ng/mL) for 10 min, IGF-1 (100 ng/mL) for 10 min, erlotinib (10 µM) for 60 min followed by EGF (100 ng/mL) for 10 min, erlotinib (10 µM) for 60 min followed by IGF-1 (100 ng/mL) for 10 min, aprepitant (10 µM) for 60 min. (B) A section of heatmap focusing on intracellular proteins phosphorylated by epidermal growth factor receptor (EGFR) activation. (C) List of 23 phosphoproteins whose phosphorylation increased by more than 20% upon stimulation of EGFR by EGF. Phosphorylation of 10 of these proteins (43% of the total phosphorylated upon EGF stimulation) also increased following treatment with aprepitant (marked with an asterisk). (D) Top 10 pathways determined by Ingenuity Pathway Analysis of RPPA data from control and EGF-stimulated HaCaT cells. (E). Top 10 pathways determined by Ingenuity Pathway Analysis of RPPA data from control and aprepitant-treated HaCaT cells.