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. 2019 Dec 9;6(4):114. doi: 10.3390/medicines6040114

Figure 2.

Figure 2

Visualization of EGFR phosphorylation at Y1068 by Western blotting. (A) HaCaT cells were treated with different concentrations of aprepitant. Western blot analysis showed that aprepitant stimulated the phosphorylation of EGFR in a dose-dependent manner. (B) Normal Human Epidermal Keratinocytes (NHEK) cells were treated with different concentrations of aprepitant. Western blot analysis showed that aprepitant increased the phosphorylation of EGFR in primary keratinocytes in a dose-dependent manner, similar to that seen in HaCaT cells.