Figure 1.

The effect of P. viscosa extract and compounds on wound healing. (a) The rate of gap closure in cultured human dermal fibroblasts (scratch assay—in vitro model of wound healing) at 0, 10, 20, 24, and 34 h after wound generation. Control was untreated fibroblasts. Crude extract and diosmin were added at a concentration of 50 µg/mL. The combination of tested compounds included diosmin at a concentration of 50 µg/mL, 1-octen-3-ol at a concentration of 10 µg/mL, and himachala-2,4-diene at a concentration of 10 µg/mL. Data from three independent experiments are shown (mean ± SD). p < 0.001. (b) The effect of diosmin (50 µg/mL) and of the combination diosmin (50 µg/mL), 1-octen-3-ol (10 µg/mL) and himachala-2,4-diene (10 µg/mL) on wound healing process in mice. Full-thickness excisional wounds were lubricated daily with cream, containing diosmin or the combination, described above. The wounds of control mice were lubricated with vehicle cream that contains all compounds except diosmin and the combination. The figure is based on the wound area on day 5. * p < 0.05.