Essential roles of VPA1701 in the in vivo colonization and motility of V. parahaemolyticus. (A) The infant rabbit received WT and ∆VPA1701 orogastrically, and the infant rabbit was killed to obtain the colonization data. The results are shown as the mean ± SD (n = 6); ** p < 0.05. (B) V. parahaemolyticus WT, ∆VPA1701, and VPA1701+ were cultured in semi-solid plates (LB with 0.3% Agar) at 37 °C and solid plates (BHI with 1.5% Agar) at 30 °C to observe the swimming and swarming ability separately. (C) The generation of the lateral flagellum was observed by TEM of WT, ∆VPA1701, and VPA1701+.