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. 2019 Oct 17;7(4):153. doi: 10.3390/vaccines7040153

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mouse sera cross-reactivity with whole N. gonorrhoeae organisms. (A) Immunoblot of FF N. gonorrhoeae strains F62, FA1090, U80401 and U80402 (2–4 × 10⁸ total organisms/slot) with pooled immune sera (wk 6) to each candidate (1:200 dilution). (B) SDS-PAGE and Coomassie staining of bacteria as in (A). (C) Whole bacteria ELISA. Each panel shows the total IgG (μg/mL ± SEM) measured in each mouse antisera (triplicate pooled sera aliquots, week 6, 1:100) against FF N. gonorrhoeae strains F62 (black bars), FA1090 (gray bars), U08401 (striped bars) and U08402 (dotted bars). Preimmune sera (white bars). *, **, ***, **** p significant (0.05 to <0.0001) by one-way ANOVA with Tukey’s multiple comparisons test (boxed asterisks = significance for all samples).