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Primers and probes used to detect the mRNA expression of the biomarkers analyzed in the study.
| Target Gene | Source | |
|---|---|---|
| CDKN2A | F: 5′-CATAGATGCCGCGGAAGGT-3′ | Life Technologies |
| R: 5′-CCCGAGGTTTCTCAGAGCCT-3′ | ||
| P: FAM-CCTCAGACATCCCCGATTGAAAGAACC-TAMRA | ||
| MKI67 | MKI67 (Hs010332443_m1 | Life Technologies |
| TOP2A | TOP2A (Hs03063307_m1) | Life Technologies |
| GUSB | GUSB (Hs99999908_m1) | Life Technologies |
| PGK1 | PGK1 (Hs99999906_m1) | Life Technologies |
