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. 2019 Dec 14;7(4):216. doi: 10.3390/vaccines7040216

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of inhibitors on the pro-inflammatory cytokine secretion induced by PotD based on ELISA. Signaling pathway inhibitors SB203580 (p38–MAPK inhibitor), U0126 (Erk1/2 inhibitor), SP600125 (JNK inhibitor), and PDTC (NF–κB inhibitor) were used to block their corresponding MAPK and NF–κB signal pathways. Protein levels of IL–1β (A), IL–6 (B), TNF–α (C) in the culture supernatants were determined by ELISA. These experiments were repeated 3 times. Each error bar represents one standard deviation from the mean.

Effects of inhibitors on the pro-inflammatory cytokine secretion induced by PotD based on ELISA. Signaling pathway inhibitors SB203580 (p38–MAPK inhibitor), U0126 (Erk1/2 inhibitor), SP600125 (JNK inhibitor), and PDTC (NF–κB inhibitor) were used to block their corresponding MAPK and NF–κB signal pathways. Protein levels of IL–1β (A), IL–6 (B), TNF–α (C) in the culture supernatants were determined by ELISA. These experiments were repeated 3 times. Each error bar represents one standard deviation from the mean.