Figure 3.

Time-kill curves for carvacrol and cinnamaldehyde treatment of five-day-old stationary phase B. henselae in comparison with control drugs. Carvacrol, cinnamaldehyde or control antibiotics were added to the stationary phase culture respectively at time point 0, and at different times of drug exposure (day 1, day 3, day 5, and day 7), portions of bacteria were removed and washed and plated on Columbia blood agar for CFU counts. The concentrations of carvacrol and cinnamaldehyde used were 0.01% (v/v) and 0.005% (v/v). Drug concentrations used in this experiment were based on their Cmax and were as follows: 2.4 μg/mL doxycycline, 10 μg/mL gentamicin, 7.8 μg/mL rifampin, 60 μg/mL daptomycin, 2.9 μg/mL methylene blue, and 6.3 μg/mL miconazole.