Table 4.
MLST genes: elongation factor 1 (ef1), beta tubulin (tubb), RNA polymerase large subunit 1 (Rpb1), beta actin (actb), elongation factor 2 (ef2) and succinate dehydrogenase complex flavoprotein subunit A (sdha) with the primer sequences and amplicon length of the fragments used in the analysis.
| Gene | Sense Primer Sequence 5′-3 | Antisense Primer Sequence | Amplicon Length (bp) |
|---|---|---|---|
| ef1 Sense | AGAAGGAAGCCGCCGATATG | GACAACCATACCGGGCTTCA | 558 |
| ef2 Sense | GAGGAGTACGCCCAAATCCC | CCATAGATACCACCACGGGC | 480 |
| Rpb1 Sense | GCTGAGGATCGACCCCAAAA | CGCGACGTATCTCTGAAGCT | 512 |
| bact Sense | CATCTATGAGGGTTATG | GATGATCTTGATCTTCA | 375 |
| tubb Sense | CTTTGTCCCTCCACCAGCTT | CGCTGGACTTTTGTTGGAGC | 378 |
| sdha Sense | GGTGGTATTACTGGACGACATCT | GGCAGAGATTGGAAGGAA | 340 |