Figure 3.

Detection of neutralizing antibody titers in immunized mouse sera. Sera collected at seven days following the second dose of EDIII were tested by a plaque reduction neutralization test (PRNT) assay for neutralizing antibodies against ZIKV human strains R103451 (A), PAN2016 (B), and PRVABC59 (C). PBS shows the background level of neutralizing antibodies. Neutralizing activity for the indicated adjuvants is presented as the neutralizing antibody titer based on the serum neutralizations at a 50% plaque reduction (PRNT₅₀). Data are expressed as the mean ± s.e.m (n = 5). Significant differences (*: p < 0.05; **: p < 0.01; ***: p < 0.001) among different groups are shown.