Figure 5.

Detection of ZIKV titers in challenged sera (A), eye (B), heart (C), spleen (D), muscle (E), and brain (F). Sera and tissues were collected at three days post-challenge with ZIKV (strain R103451), and viral load determined by a plaque-forming assay. Mice immunized with PBS served as the control. The detection limits for the samples were ~20 PFU/mL (sera), ~50 PFU/g (eye), and ~25 PFU/g (heart, spleen, muscle, and brain). Data are expressed as the mean ± s.e.m (n = 5). Significant differences (*: p < 0.05; **: p < 0.01; ***: p < 0.001) among different groups are shown.