Figure 6.

Detection of ZIKV RNA in challenged sera (A), eye (B), heart (C), spleen (D), muscle (E), and testis (F). Sera and tissues were collected at three days post-challenge with ZIKV (strain R103451) and viral RNA copies determined by qRT-PCR. Mice immunized with PBS served as the control. The detection limits were ~10³ RNA copies/mL (sera), ~5 × 10² RNA copies/g (eye, heart, and spleen), and ~2.5 × 10² RNA copies/g (muscle and testis). Data are expressed as the mean ± s.e.m (n = 5). Significant differences (*: p < 0.05; **: p < 0.01; ***: p < 0.001) among different groups are shown.