Figure 1.

Antibody dose-dependent response in mice. Mice were immunized intramuscular (IM) (A) and intranasal (IN) (B) with serial dilutions of rAd-gag vaccine expressing the HIV-1 gag gene. Three weeks after immunization, the mice were bled and antibodies were determined using an ELISA. Sera were diluted 1:100 in PBS (1% BSA) and incubated on plates coated with HIV-1 gag protein. The mouse antibodies were detected using an anti-mouse H + L HRP polyclonal secondary antibody. HRP was detected using the 1-Step Ultra TMB-ELISA (Thermo Fisher), the reaction was stopped with 2 M sulfuric acid and the OD450 was determined. (* p < 0.05; ** p < 0.01; *** p < 0.001).