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. 2019 Nov 9;8(4):227. doi: 10.3390/pathogens8040227

Figure 4.

Figure 4

Molecular detection of P. tolaasii using DNA template isolated from three selected colonies (Lanes 1, 2, and 3) growing on PAF agar. Amplified products were obtained in a nested PCR assay. Profile A is the first-round PCR product (449 bp) with primer set Pt-1A/Pt-1D1, and B second-round PCR product (249 bp) with the internal primer set Pt-PM/Pt-QM as described in Lee et al. [36]. They were separated in agarose gel electrophoresis. Lane M, Trans2K DNA Marker (TransGen Ltd., China). DNA fragments of 449 bp and 249 bp indicate the presence of P. tolaasii.