Table 2.
UHPLC-DAD-MS/MS data of compounds detected in analyzed extracts.
| Peak No. | Compound Name | Retention Time (min) | UV (nm) | (M − H)− m/z | MS2 ions | (M + H)−+ m/z | MS2 ions | Compound Content ug/mg | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Extract I 20% EtOH | Extract II 60% EtOH | Extract III 96% EtOH | ||||||||
| 1 | Monotropeint | 2.1 | 237 | 389 | 369, 227, 209b, 183, 137 | 381 | - | n.q. | n.q. | n.q. |
| 2 | 10-Desacetylasperulosidic acidt | 3.7 | 237 | 389 | 227b, 209, 183 | 381 | - | n.q. | n.q. | n.q. |
| 3 | p-Hyroxybenzoic acids | 5.9 | 259, 294 | 153 | - | 155 | - | n.q. | n.q. | n.q. |
| 4 | 3-O-Caffeoylquinic acids | 9.7 | 217, 241, 300sh, 324 | 353 | 191b, 179, 161 | 355 | 163b | 8.91 ± 0.09 | 3.72 ± 0.12 | 0.53 ± 0.03 |
| 5 | Asperulosidic acidt | 14.0 | 235 | 431 | 371, 269, 251b, 165 | 433 | - | n.q. | n.q. | n.q. |
| 6 | 5-O-Caffeoylquinic acids (Chlorogenic acid) | 16.2 | 219, 241, 299sh, 325 | 353 | 191b, 179 | 355 | 163b | 18.44 ± 0.21 | 31.51 ± 0.19 | 40.61 ± 0.12 |
| 7 | 4-O-Caffeoylquinic acids | 18.2 | 217, 241, 300sh, 325 | 353 | 191, 179, 173b | 355 | 337, 307, 163b | 8.12 ± 0.03 | 4.75 ± 0.08 | 1.16 ± 0.02 |
| 8 | Quercetin 3-O-rhamnoglucoside-7-O-glucosides | 23.1 | 255, 263sh, 353 | 771 | 609b, 301 | 773 | 627, 611, 465b, 303 | 3.35 ± 0.10 | 2.95 ± 0.05 | 1.50 ± 0.02 |
| 9 | Quercetin 3-O-rhamnoglucosides (Rutin) | 36.9 | 255, 262sh, 354 | 609 | 591, 301b, 179 | 611 | 465, 303b | 1.08 ± 0.05 | 2.49 ± 0.03 | 6.33 ± 0.07 |
| 10 | Caffeic acid derivative | 38.0 | 254, 299sh, 327 | 381 | 207, 191, 179b, 135 | 383 | 365, 163b | n.q. | n.q. | n.q. |
| 11 | Isorhamnetin 3-O-glucorhamnosides | 40.4 | 259, 260sh, 350 | 615 | 609b, 542, 461, 315 | 617 | - | n.q. | n.q. | n.q. |
| 12 | 3,4-O-Dicaffeoylquinic acids | 42.9 | 239, 300sh, 324 | 515 | 353b, 255, 173b | 517 | 499b, 317, 163 | n.q. | n.q. | n.q. |
| 13 | 3,5-O-Dicaffeoylquinic acids | 43.8 | 240, 299sh, 324 | 515 | 353, 233, 191b, 179 | 517 | 499b, 147 | 0.23 +/− 0.02 | 0.42 +/− 0.08 | 2.43 +/− 0.11 |
| 14 | 4,5-O-Dicaffeoylquinic acids | 47.9 | 240, 300sh, 325 | 515 | 515, 353b, 299, 255, 203 | 517 | 499, 335b, 278 | n.q. | n.q. | n.q. |
s—comparisons with chemical standard have been made; b—base peak (the most abundant ion in recorded spectrum); sh-shoulder; t—tentative assignment; n.q.—not quantified.