Figure 2.

Immune responses elicited by MVA-ZIKV vaccines. BALB/c mice (n = 6) were intramuscularly immunised with a single dose of MVA encoding ZIKV antigens at 1 × 10⁶ plaque forming units (PFU)/mouse. Serum samples were collected at 4 weeks and 12 weeks post immunisation. (a) Antibody responses elicited by MVA-ZIKV vaccine candidates at 4 weeks and (b) 12 weeks post immunisation were quantified by ELISA in plates coated with a ZIKV E protein [19]. (c) Cellular immune responses to MVA-ZIKV vaccine candidates. PBMCs IFNγ–producing cells after 4 weeks post immunisation were measured by (IFNγ) ex vivo ELISPOT, and 20-mer peptides spanning the ZIKV prME proteins at 10 μg/mL were used for stimulation. p-values were determined by one-way ANOVA and Tukey’s multiple comparisons test.